长聘教轨副教授
致力于打破昂贵辅因子在生物催化工业化应用中的成本瓶颈，通过开发化学-酶法耦合（Chemoenzymatic）的一锅法反应体系，实现了S-腺苷甲硫氨酸（SAM）等关键辅因子的高效原位再生。特别是在依赖钴胺素（Cobalamin，即维生素B12）的甲基转移酶反应中，成功利用廉价底物（如甲醇）作为驱动力，实现了金属有机辅因子的可持续循环利用，显著降低生物合成的经济成本
针对许多高效生物催化剂（特别是B12依赖型酶）易受氧气氧化而失活的难题，专注于开发在常温、有氧环境（Ambient）下稳定操作氧敏感酶的创新技术。通过设计特殊的反应路径和保护机制，使原本必须在严格厌氧条件下进行的反应能够在常规环境下进行，极大简化操作流程，为氧敏感微生物和酶制剂的工业化应用扫清障碍
聚焦于利用合成生物学手段，解决氧敏感酶在异源宿主（如 E. coli Nissle）中活性表达难的问题，并探索其实际应用价值。研究通过构建多酶级联反应（Multi-enzyme Cascade），将这些功能性表达的酶应用于生物质的高值化利用。具体成果包括将木糖（Xylose）转化为NAD(P)H，以及利用嗜热蛋白酶级联技术将豆渣（Soybean pulp）转化为氨基酸和蛋白质，实现从农业/工业废弃物到高附加值产品的绿色生物制造
Wang, P. H., Wu, T. Y., Chen, Y. L., Gicana, R. G., Lee, T. H., Chen, M. J., Hsiao, T. H., Lu, M. Y. J., Lai, Y. L., Wang, T. Y., Li, J. Y., & Chiang, Y. R. (2025). Bacterial estrogenesis without oxygen: Wood-Ljungdahl pathway likely contributed to the emergence of estrogens in the biosphere. Proceedings of the National Academy of Sciences of the United States of America, 122(10).
Ng, I. S., Wang, P.-H., Ting, W.-W., & Juo, J.-J. (2025). Recent progress in one-pot enzymatic synthesis and regeneration of high-value cofactors. Trends in Biotechnology, 43(2), 270-273.
Wang, P.-H., Chen, Y.-L., Wei, S. T.-S., Wu, K., Lee, T.-H., Wu, T.-Y., & Chiang, Y.-R. (2019). Retroconversion of estrogens into androgens by bacteria via a cobalamin-mediated methylation. Proceedings of the National Academy of Sciences of the United States of America, 117(3), 1395-1403.
Nishikawa, S., Yu, W. C., Chiang, Y. R., Wu, T. Y., Tang, Khusnutdinova, A., Yakunin, A., Fujishima, K., & Wang, P. H. (2025). Methanol-Driven, One-Pot Chemoenzymatic-Adenosylmethionine Regeneration for Ambient Cobalamin-Dependent Methyltransferase Reactions. ACS Catalysis, 15(15), 13689-13703.
Nishikawa, S., Yu, W. C., Jia, T. Z., He, M. J., Khusnutdinova, A., Yakunin, A. F., Chiang, Y. R., Fujishima, K., & Wang, P. H. (2024). Amino Acid Self-Regenerating Cell-Free Protein Synthesis System that Feeds on PLA Plastics, CO, Ammonium, and α-Ketoglutarate. ACS Catalysis, 14(10), 7696-7706.
Lin, Y. H., Nishikawa, S., Jia, T. Z., Yeh, F. I., Khusnutdinova, A., Yakunin, A. F., Fujishima, K., & Wang, P. H. (2023). One-pot chemo-enzymatic synthesis and one-step recovery of length-variable long-chain polyphosphates from microalgal biomass. Green Chemistry, 25(23), 9896-9907.
Wang, P. H., Nishikawa, S., McGlynn, S. E., & Fujishima, K. (2023). One-Pot Synthesis of [4Fe-4S] Proteins Using a Recombinant SUF System under Aerobic Conditions. ACS Synthetic Biology, 12(10), 2887-2896.
Wang, P. H., Khusnutdinova, A. N., Luo, F., Xiao, J., Nemr, K., Flick, R., Brown, G., Mahadevan, R., Edwards, E. A., & Yakunin, A. F. (2018). Biosynthesis and Activity of Prenylated FMN Cofactors. Cell Chemical Biology, 25(5), 560-+.
Yan, J., Bi, M., Bourdon, A. K., Farmer, A. T., Wang, P.-H., Molenda, O., Quaile, A. T., Jiang, N., Yang, Y., Yin, Y., Şimşir, B., Campagna, S. R., Edwards, E. A., & Löffler, F. E. (2017). Purinyl-cobamide is a native prosthetic group of reductive dehalogenases. Nature Chemical Biology, 14(1), 8-14.
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